Description
JC-1 is a fluorescent lipophilic carbocyanine dye, which can be used to measure mitochondrial membrane potential.A dual-emission potential-sensitive probe that can be used to measure mitochondrial membrane potential. JC-1 is a green-fluorescent (λex 520 nm) monomer at low membrane potential. At higher potentials, JC-1 forms red-fluorescent (λem 596 nm ) "J-aggregates," which exhibit broad excitation and very narrow emission spectra. The ratio of red to green fluorescence of JC-1 is dependent only on membrane potential, and not influenced by mitochondrial size, shape, or density.The decrease of mitochondrial membrane potential is a landmark event in the early stage of apoptosis. The decrease of cell membrane potential can be easily detected by the transition of JC-1 from red fluorescence to green fluorescence, and the transition from red fluorescence to green fluorescence of JC-1 can also be used as an early detection indicator of apoptosis.The maximum excitation wavelength of JC-1 monomer is 514nm and the maximum emission wavelength is 529nm; the maximum excitation wavelength of JC-1 polymer is 585nm and the maximum emission wavelength is 590nm. For actual observation, use the conventional settings for observing red fluorescence and green fluorescence.The commonly used concentration range of JC-1 for detecting the mitochondrial membrane potential of cells is 1-20µg/mL, and the suitable concentration of JC-1 for many cells is 10µg/mL.PrecautionsIf the amount of JC-1 used at a time is small, each tube needs to be appropriately divided to avoid repeated freeze-thaw.For your safety and health, please wear lab coats and disposable gloves